Generator

Part:BBa_K228869:Design

Designed by: Haoqian Zhang   Group: iGEM09_PKU_Beijing   (2009-09-22)


AraC(K228009) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.


Source

from 2009 part registry distribution & Peking University 2009 igem team

References